Effect of Green Selenium Nanosynthesis on Staphylococcus Aureus

Ali, Hassan; Alzubadiy, Asmaa

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Proceedings of International Conference on Applied Innovation in IT · 2025/06/27 · Vol. 13 · Issue 2 · pp. 873–879

Effect of Green Selenium Nanosynthesis on Staphylococcus Aureus

Hassan Naser Ali, Asmaa M. Salih Almohaidi and Mohanad W. Mahdi Alzubadiy

📄 Download PDF DOI: <a href="http://dx.doi.org/10.25673/120617">10.25673/120617</a>

Abstract

Selenium nanoparticles (SeNPs) represent a promising natural compound for modern medical treatments, offering a potent new weapon against pathogenic bacteria. This study aimed to synthesize SeNPs using sodium selenite (Na₂SeO₃) as a precursor and dried Camellia sinensis (green tea) leaf extract as a reducing and stabilizing agent through an eco-friendly biosynthesis approach. The synthesis was performed via a simple, cost-effective, and environmentally friendly method. The resulting nanoparticles were characterized using UV-visible spectroscopy, field-emission scanning electron microscopy (FE-SEM), and energy-dispersive X-ray spectroscopy (EDX). FE-SEM analysis confirmed the formation of spherical SeNPs with varied shapes and an average particle size of 20.5 nm, alongside a narrow size distribution. While the antibacterial evaluation against Staphylococcus aureus showed no significant inhibitory activity against planktonic cells, the SeNPs exhibited a strong antibiofilm effect, inhibiting biofilm formation by 54.7%. These findings highlight the potential of biosynthesized SeNPs as a specific and effective anti-biofilm agent for targeting bacterial biofilms and combating antibiotic resistance.

Keywords

SeO Nanoparticles Anti-Bacterial Antibiofilm Staphylococcus Aureus.

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