Proceedings of International Conference on Applied Innovation in IT
2025/06/27, Volume 13, Issue 2, pp.819-823
Early Diagnosis of Fetal Sex and its Relationship to Some Biochemical and Physiological Variables in Pregnant Women in Salah Al-Din Governorate
Noor Lateef Omer and Hussien Mohammad Tayawi Abstract: Genes for sex determination are widely analyzed and used to predict fetal sex during different trimesters of pregnancy. However, their correlation with sex prediction based on ultrasound results, as well as with testosterone and estrogen levels in the first trimester, is quite limited. Therefore, the present study aimed to assess the degree of agreement between several sex-determining genes (SRY, DYS14, and DAZ) analyzed in the first trimester and Doppler-predicted sex, and to evaluate their correlation with hormone levels.Whole blood samples (5 mL) were collected from 110 Iraqi pregnant women between the 1st and 12th weeks of pregnancy. DNA was extracted from all samples, and the presence of the selected genes was confirmed using real-time PCR. The detection of amplification was considered a positive result for each gene, indicating male sex. The concentrations of testosterone and estrogen were measured using the ELISA technique. All pregnancies were monitored to confirm the Doppler results postnatally. The results of the present study showed no significant correlations between sex predicted by the SRY, DYS14, and DAZ genes and sex predicted by Doppler. Furthermore, the levels of both hormones did not significantly correlate with sex predicted by either molecular or Doppler methods. In conclusion, sex prediction in the first trimester of pregnancy frequently yields inaccurate results.
Keywords: SRY, DYS14, DAZ, Doppler, Real Time PCR, Testosterone, Estrogen, Iraq.
DOI: Under Indexing
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References:
- T. Gamble and D. Zarkower, “Sex determination,” Current Biology, vol. 22, no. 8, pp. R257–R262, 2012, doi: 10.1016/j.cub.2012.02.054.
- C. Colmant, M. Morin-Surroca, F. Fuchs, H. Fernandez, and M. V. Senat, “Non-invasive prenatal testing for fetal sex determination: is ultrasound still relevant?,” European Journal of Obstetrics & Gynecology and Reproductive Biology, vol. 171, no. 2, pp. 197–204, 2013, doi: 10.1016/j.ejogrb.2013.09.027.
- M. Bakker, E. Birnie, P. Robles de Medina, K. M. Sollie, E. Pajkrt, and C. M. Bilardo, “Total pregnancy loss after chorionic villus sampling and amniocentesis: a cohort study,” Ultrasound in Obstetrics & Gynecology, vol. 49, no. 5, pp. 599–606, 2017, doi: 10.1002/uog.15983.
- S. M. Gelaw and H. Bisrat, “The role of ultrasound in determining fetal sex,” Ethiopian Journal of Health Development, vol. 25, no. 3, pp. 216–221, 2011.
- S. Manzanares, A. Benítez, M. Naveiro‐Fuentes, M. S. López‐Criado, and M. Sánchez‐Gila, “Accuracy of fetal sex determination on ultrasound examination in the first trimester of pregnancy,” Journal of Clinical Ultrasound, vol. 44, no. 5, pp. 272–277, 2016, doi: 10.1002/jcu.22320.
- R. J. P. Rijnders, R. B. Van Der Luijt, E. D. J. Peters, J. K. Goeree, C. E. Van Der Schoot, J. K. Ploos Van Amstel, and G. C. M. L. Christiaens, “Earliest gestational age for fetal sexing in cell‐free maternal plasma,” Prenatal Diagnosis, vol. 23, no. 13, pp. 1042–1044, Dec. 2003, doi: 10.1002/pd.748.
- S. Drury, M. Hill, and L. S. Chitty, “Cell-free fetal DNA testing for prenatal diagnosis,” Advances in Clinical Chemistry, vol. 76, pp. 1–35, 2016, doi: 10.1016/bs.acc.2016.05.001.
- K. A. Sillence, L. A. Roberts, H. J. Hollands, H. P. Thompson, M. Kiernan, T. E. Madgett, et al., “Fetal sex and RHD genotyping with digital PCR demonstrates greater sensitivity than real-time PCR,” Clinical Chemistry, vol. 61, no. 11, pp. 1399–1407, Nov. 2015, doi: 10.1373/clinchem.2015.240366.
- J. W. Wang, Y. N. Lyu, B. Qiao, Y. Li, Y. Zhang, P. K. Dhanyamraju, et al., “Cell-free fetal DNA testing and its correlation with prenatal indications,” BMC Pregnancy and Childbirth, vol. 21, no. 1, p. 585, 2021, doi: 10.1186/s12884-021-04092-6.
- Y. Zhou, Z. Zhu, Y. Gao, Y. Yuan, Y. Guo, L. Zhou, et al., “Effects of maternal and fetal characteristics on cell-free fetal DNA fraction in maternal plasma,” Reproductive Sciences, vol. 22, no. 11, pp. 1429–1435, Nov. 2015, doi: 10.1177/1933719115589414.
- P. Hercz, Z. Kazy, P. Siklos, and L. Ungar, “Quantitative comparison of serum steroid and peptide hormone concentrations in male and female fetuses in the maternal-fetoplacental system during the 28th–40th weeks of pregnancy,” European Journal of Obstetrics & Gynecology and Reproductive Biology, vol. 30, no. 3, pp. 201–204, 1989, doi: 10.1016/0028-2243(89)90168-8.
- H. Schock, A. Zeleniuch-Jacquotte, E. Lundin, K. Grankvist, H. Å. Lakso, A. Idahl, et al., “Hormone concentrations throughout uncomplicated pregnancies: a longitudinal study,” BMC Pregnancy and Childbirth, vol. 16, no. 146, pp. 1–11, 2016, doi: 10.1186/s12884-016-0937-5.
- J. Wuu, S. Hellerstein, L. Lipworth, L. Wide, B. Xu, G. P. Yu, et al., “Correlates of pregnancy oestrogen, progesterone and sex hormone-binding globulin in the USA and China,” European Journal of Cancer Prevention, vol. 11, no. 3, pp. 283–293, 2002, doi: 10.1097/00008469-200206000-00008.
- A. T. Toriola, M. Vääräsmäki, M. Lehtinen, A. Zeleniuch-Jacquotte, E. Lundin, K. G. Rodgers, et al., “Determinants of maternal sex steroids during the first half of pregnancy,” Obstetrics & Gynecology, vol. 118, no. 5, pp. 1029–1036, Nov. 2011, doi: 10.1097/AOG.0b013e318233a985.
- I. Y. Järvelä, S. Pelkonen, O. Uimari, K. Mäkikallio, K. Puukka, A. Ruokonen, and H. Martikainen, “Controlled ovarian hyperstimulation leads to high progesterone and estradiol levels during early pregnancy,” Human Reproduction, vol. 29, no. 11, pp. 2393–2401, 2014, doi: 10.1093/humrep/deu196.
- H. R. K. Khorshid, M. Zargari, M. R. Sadeghi, H. Edallatkhah, M. H. Shahhosseiny, and K. Kamali, “Early fetal gender determination using real-time PCR analysis of cell-free fetal DNA during 6th–10th weeks of gestation,” Acta Medica Iranica, vol. 51, no. 4, pp. 209–214, 2013.
- Y. D. Lo, P. Patel, M. Sampietro, M. D. G. Gillmer, K. A. Fleming, and J. S. Wainscoat, “Detection of single-copy fetal DNA sequence from maternal blood,” The Lancet, vol. 335, no. 8703, pp. 1463–1464, 1990, doi: 10.1016/0140-6736(90)91350-A.
- J. Chi, J. A. Hyett, K. M. Finning, C. A. Lee, and R. A. Kadir, “Non‐invasive first trimester determination of fetal gender: a new approach for prenatal diagnosis of haemophilia,” BJOG: An International Journal of Obstetrics & Gynaecology, vol. 113, no. 2, pp. 239–242, 2006, doi: 10.1111/j.1471-0528.2005.00823.x.
- V. Mazza, C. Falcinelli, A. Percesepe, S. Paganelli, A. Volpe, and A. Forabosco, “Non‐invasive first trimester fetal gender assignment in pregnancies at risk for X‐linked recessive diseases,” Prenatal Diagnosis, vol. 22, no. 10, pp. 919–924, Oct. 2002, doi: 10.1002/pd.440.
- J. A. Hyett, G. Gardener, T. Stojilkovic‐Mikic, K. M. Finning, P. G. Martin, C. H. Rodeck, and L. S. Chitty, “Reduction in diagnostic and therapeutic interventions by non‐invasive determination of fetal sex in early pregnancy,” Prenatal Diagnosis, vol. 25, no. 12, pp. 1111–1116, 2005, doi: 10.1002/pd.1250.
- C. D. Martinhago, R. M. de Oliveira, M. D. C. Tomitao Canas, L. D. Vagnini, J. B. Alcantara Oliveira, C. G. Petersen, and J. G. Franco Junior, “Accuracy of fetal gender determination in maternal plasma at 5 and 6 weeks of pregnancy,” Prenatal Diagnosis, vol. 26, no. 13, pp. 1219–1223, 2006, doi: 10.1002/pd.1556.
- F. M. Liu, X. Y. Wang, X. Feng, W. Wang, Y. X. Ye, and H. Chen, “Feasibility study of using fetal DNA in maternal plasma for non-invasive prenatal diagnosis,” Acta Obstetricia et Gynecologica Scandinavica, vol. 86, no. 5, pp. 535–541, 2007, doi: 10.1080/00016340601185488.
- M. Hill, K. Finning, P. Martin, J. Hogg, C. Meaney, G. Norbury, et al., “Non‐invasive prenatal determination of fetal sex: translating research into clinical practice,” Clinical Genetics, vol. 80, no. 1, pp. 68–75, 2011, doi: 10.1111/j.1399-0004.2010.01526.x.
- Y. D. Lo, M. S. Tein, T. K. Lau, C. J. Haines, T. N. Leung, P. M. Poon, et al., “Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis,” The American Journal of Human Genetics, vol. 62, no. 4, pp. 768–775, 1998, doi: 10.1086/301800.
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