Proceedings of International Conference on Applied Innovation in IT
2025/06/27, Volume 13, Issue 2, pp.607-613
Determination of Tetracycline Hydrochloride by Using Dapsone as a Reagent in Developed Spectrophotometric Analysis
Amal Y. Manna and Mohsen H. Bakir Abstract: Inexpensive spectrophotometric method has been developed to estimate its pure concentration. This method is based on the azo coupling reaction using Diaz onium dapsone, which is formed in a solution containing hydrochloric acid and sodium nitrate. The resulting salt reacts with tetracycline hydrochloride to form a Tetracycline hydrochloride is considered an antibiotic, and a sensitive, easy, fast, and orange azo dye at a wavelength of 380 nanometres. After optimizing the reaction conditions, a recovery rate of 100% was achieved, with a Beer-Lambert limit between (2 - 26) µg /mL, and a Sandal sensitivity of 0.0238 µg.cm². The method also demonstrated high sensitivity without any interference from the excipients present in the formulation, with a sensitivity value of 20160 L·mol⁻¹·cm⁻¹ and a correlation coefficient of 0.9992. The limit of detection (LOD) was found to be 0.094 and the limit of quantification (LOQ) was found to be 0.28. The reaction was stable for a sufficient period, making the method suitable for estimating tetracycline in its pure form and in pharmaceutical preparations, such as capsules, without any interference.
Keywords: Spectrophotometric, Dapson, Diazotization, Tetracycline Hydrochloride.
DOI: 10.25673/120548
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