Proceedings of International Conference on Applied Innovation in IT  ·  2026/03/31  ·  Vol. 14  ·  Issue 1  ·  pp. 933–943
The Prevalence of Integron Class I and Class II Among Carbapenem Resistance Klebsiella Pneumonia: A Cross-Sectional Study
Ebtehal Mohammed Altamimi, Abbas Yaseen Hasan and Izdehar Mohammed Jasim
📄 Download PDF DOI: 10.25673/123656
Abstract
The global frequency of Carbapenem-resistant Klebsiella pneumoniae (CRKP) continues to rise, causing the World Health Organization to declare it a critical threat to the health of public. CRKP was regarded as a challenging organism to treat due to restricted therapy alternatives. This study aimed to determine the antibiotics resistance patterns and the prevalence of Integron I, II, and their associated genes in K. pneumoniae isolated from various clinical specimens. Overall, 300 clinical samples obtained from (blood, urine, sputum, burns, and wounds), fifty (16.66%) isolates were identified as K.pneumonia. Out of 50 isolates. Antimicrobial sensitivity test were performed for all Fifty isolates of K. pneumonia,16(32%) from isolates have been resistance to carbapenem. The prevalence of K. pneumoniae isolates by origin was 11 (22%) from urine. 15(30%) wound, 10(20%) from sputum, 11(22%) from was burns and only 3(6%) blood, The isolates were examined for class I and II integrons as well as carbapeneme resistance genes using PCR. Antibiotics sensitivity indicated all of the isolates 50(100%) were resistance to piperacillin/tazobactam,49(98%) resistance to cefoxitin, and the lowest resistance against to chloramphenicol. Amplification of PCR products revealed that 12 (75%) of the isolates exhibited class I (intl1) integrons, whereas 8 (50%) displayed class II (intl2) integrons, blaIMP-1 was the most prevalent carbapenem resistance gene 10 (62.5%), followed by blaNDM 6(37.5%), blaOXA-48 5(31.25%), blaIMP-2 3(18.75%), blaVIM-2 2(12.5%) and blaKPC-1 1(6.25%). 8 hyper-virulence strains tested positively to blaOXA-48, while 2 had blaNDM genes.
Keywords
Klebsiella Pneumoniae Carbapenem-Resistant Genes Integrons Antibiotic.
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