Proceedings of International Conference on Applied Innovation in IT  ·  2026/03/31  ·  Vol. 14  ·  Issue 1  ·  pp. 913–921
Computational Analysis of ABCC8 and KCNJ11 Gene Variants in Type 1 Diabetes
Salsabeel Mohammed Abdallha, Yasir Adil Jabbar Alabdali and Mohammed Qasim Waheeb
📄 Download PDF DOI: 10.25673/123654
Abstract
Type 1 diabetes (T1D) is an autoimmune disease characterized by progressive destruction of pancreatic β-cells and chronic hyperglycemia. Genetic variants in ATP-sensitive potassium (K-ATP) channel genes, including ABCC8 and KCNJ11, may influence insulin secretion and susceptibility to diabetes. This study aimed to evaluate the association of ABCC8 rs1799854, ABCC8 rs1801261, and KCNJ11 rs5219 polymorphisms with T1D risk and related clinical and biochemical parameters in children. A case-control design was used, including 60 T1D patients and 60 age- and sex-matched healthy controls recruited between November 2024 and February 2025. Genomic DNA was extracted from peripheral blood, and genotyping was performed using PCR-RFLP for ABCC8 variants and ARMS-PCR for KCNJ11. Clinical and biochemical parameters, including BMI, lipid profile, HbA1c, and random blood glucose, were measured using standard automated methods. Statistical analysis included chi-square tests, t-tests, and ANOVA, with odds ratios (ORs) and 95% confidence intervals (CIs) used to estimate associations. The heterozygous CT genotypes of ABCC8 rs1799854 and KCNJ11 rs5219 were significantly associated with increased T1D risk (OR = 3.27, p = 0.003; OR = 6.10, p < 0.0001). In contrast, the ABCC8 rs1801261 TT genotype showed a protective association with T1D (OR = 0.22, p = 0.013). Significant genotype-related differences were observed in BMI, HDL cholesterol, HbA1c, and glucose levels. These findings suggest that ABCC8 and KCNJ11 polymorphisms may contribute to T1D susceptibility and metabolic variation in affected children. However, larger studies are required to confirm these associations.
Keywords
ABCC8 Gene KCNJ11 Gene T1D Lipid Profile.
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