Statistical Evaluation of the Biological Effects of Desert Locust Extract Via Dose-Response Curves In Esophageal Cancer Cells

Mohammed Nazar Hussain Alagele and Ammar Ahmed Sultan

References:

1. A. Saeed, A. Jabeen, I. Mushtaq, and I. Murtaza, “Cancer: Insight and Strategies to Reduce,” Pathophysiology of Cancer: An Interdisciplinary Approach, vol. 22, p. 49, 2025.
2. J. S. Brown et al., “Updating the definition of cancer,” Mol. Cancer Res., vol. 21, no. 11, pp. 1142–1147, 2023.
3. World Health Organization, WHO Framework for Meaningful Engagement of People Living with Noncommunicable Diseases, and Mental Health and Neurological Conditions, Geneva: WHO, 2023.
4. L. Qi et al., “Global esophageal cancer epidemiology in 2022 and predictions for 2050: A comprehensive analysis and projections based on GLOBOCAN data,” Chin. Med. J., vol. 137, no. 24, pp. 3108–3116, 2024.
5. AL-Ammari, A. M., Hussain, A. F., & Ismael, T. K., “Prevalence of fungal pathogens among respiratory tract infections,” Ann. Trop. Public Health, vol. 22, no. 8, S227, 2019.
6. W. Jin et al., “Global, regional, and national burden of esophageal cancer: A systematic analysis of the Global Burden of Disease Study 2021,” Biomark. Res., vol. 13, no. 1, p. 3, 2025.
7. Y. Wang et al., “Esophageal cancer,” in StatPearls, Treasure Island, FL: StatPearls Publishing, 2024.
8. D. Zhao et al., “Multi-omics characterization of esophageal squamous cell carcinoma identifies molecular subtypes and therapeutic targets,” JCI Insight, vol. 9, no. 10, e171916, 2024.
9. W. Jiang et al., “Current status and perspectives of esophageal cancer: A comprehensive review,” Cancer Commun., vol. 45, no. 3, pp. 281–331, 2025.
10. Dobermann, D. and Scheers, N., “Cytotoxicity of insects (T. molitor, G. bimaculatus, S. gregaria and A. domesticus) in a simulated gastrointestinal digestion/human intestinal cell model,” Food Research International, vol. 143, 110276, 2021.
11. A. M. Zedan et al., “Oriental hornet (Vespa orientalis) larval extracts induce antiproliferative, antioxidant, anti-inflammatory, and anti-migratory effects on MCF7 cells,” Molecules, vol. 26, no. 11, p. 3303, 2021.
12. I. El-Garawani et al., “Enhanced antioxidant and cytotoxic potentials of lipopolysaccharides-injected Musca domestica larvae,” Pharmaceutics, vol. 12, no. 11, p. 1111, 2020.
13. F. Ramos-Martín, C. Herrera-León, and N. d’Amelio, “Molecular basis of the anticancer, apoptotic and antibacterial activities of Bombyx mori Cecropin A,” Arch. Biochem. Biophys., vol. 715, p. 109095, 2022.
14. J. E. Lee et al., “Hepatoprotective and anticancer activities of Allomyrina dichotoma larvae,” J. Life Sci., vol. 25, no. 3, pp. 307–316, 2015.
15. R. A. Wu et al., “Caspase 3-mediated cytotoxicity of mealworm larvae (Tenebrio molitor) oil extract against human hepatocellular carcinoma and colorectal adenocarcinoma,” J. Ethnopharmacol., vol. 250, p. 112438, 2020.
16. M. W. M. Alzubaidy and M. N. Hussain, “Biosynthetic of green zinc oxide nanoparticles with effect on cancer cell line HeLa,” Revis Bionatura, vol. 8, no. 2, p. 22, 2023.
17. A. F. Hussain et al., “An annotated bibliography of medical mycology in Iraq: 1962–2021,” Microb. Biosyst., vol. 6, no. 1, pp. 11–31, 2021.
18. A. A. J. Al-Karawi, N. Y. Al-Bayati, and M. A. A. Shafeeq, “Comparative Analysis of Vegetable Chemical Compounds from Orange Peel Extract and Thuja Plants Using Gas Chromatography Mass Spectrometry Technique,” in Proc. Int. Conf. Appl. Innovation IT, vol. 13, no. 2, pp. 615–622, 2025.
19. S. Wu and D. K. Shah, “Determination of ADC cytotoxicity in immortalized human cell lines,” in Antibody-Drug Conjugates, New York, NY: Humana, 2020, pp. 329–340.
20. B. M. A. Latif and M. W. M. Alzubaidy, “Effect of zinc oxide nanoparticles on activity of skin cancer cell line (A-375),” AIP Conf. Proc., vol. 2593, no. 1, p. 050003, 2023.
21. M. Y. Ahn et al., “Anti-cancer effect of dung beetle glycosaminoglycans on melanoma,” BMC Cancer, vol. 19, 2019.
22. X. Cheseto et al., “Potential of the desert locust Schistocerca gregaria as an unconventional source of dietary and therapeutic sterols,” PLoS One, vol. 10, no. 5, e0127171, 2015.