Proceedings of International Conference on Applied Innovation in IT
2025/06/27, Volume 13, Issue 2, pp.845-855

Broad-Spectrum Antibacterial Activity of the Buds Rosa Damascena Bud Extracts Against Pathogens Isolated Clinically


Hussein W. Rabeea, Furqan Fadhil Najm, Hassan Abdulreza Fayyad and Muhammed Ali Sadiq


Abstract: We present the chemical composition of the flower buds of Rosa damascena, as well as their inhibitory activity against pathogenic Gram-positive and Gram-negative bacterial strains. GC-MS analysis for the Detection of bioactive compounds in Rose Damascena buds and isolation of bacteria by using the Vitek 2 system. Evaluate the antibacterial activity of water and ethanolic extracts on multidrug-resistant strains. Assess the activity of both extracts also on bacterial growth inhibition. Methods: Preparation of Extracts: The flower buds (including calyx leaves, petals, stamens, and receptacles) were ground and extracted with water (aqueous) and ethanol (ethanolic). Antibacterial Assays: Tested extracts against seven bacterial strains using agar well diffusion method: Gram-positive: Staphylococcus aureus, Gram-negative: Escherichia coli, Klebsiella pneumoniae, Serratia, Pseudomonas aeruginosa, Proteus spp., Acinetobacter baumannii. Concentrations tested: Aqueous (15–50 mg/mL), Ethanolic (25–100 mg/mL). Bioactive Compound Profile: Bioactive compounds were identified by GC-MS. Results: The Inhibitory Activity: Ethanolic extract: Exhibited substantial inhibition against S. aureus, Serratia, and P. aeruginosa at all tested concentrations. Aqueous extract: More effective than ethanolic extract on K. pneumoniae and Proteus spp. (15–50 mg/mL). Strains that were not responsive: Both extracts did not affect A. baumannii. Isolates of E. coli were partially resistant, where one isolate was sensitive to ethanolic extract and another to aqueous extract. Chemical Composition: GC-MS analysis shows bioactive compounds such as 2,3-Butanediol, Oxirane,2,3-dimethyl and 6-Oxa-bicyclo[3.1.0]hexan-3-one are probably responsible for different antimicrobial activities. Conclusion: Rosa damascena buds are a reservoir of effective bioactive substances and have significant antibacterial potential, especially against S. aureus and P. aeruginosa. However, resistance seen in strains such as A. baumannii highlights the necessity for additional research to improve extraction strategies or to pair the active compounds with traditional antibiotics. The findings highlight the plant as a promising source of natural antimicrobials in the fight against antibiotic resistance.

Keywords: Rosa Damascene, Antibacterial Effects, Plant Extracts, Gas Chromatography-Mass Spectrometry.

DOI: Under Indexing

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References:

  1. K. Arunachalam, X. Yang, and S. P. Sasidharan, "Correction to: Bioprospecting of Tropical Medicinal Plants," in Bioprospecting of Tropical Medicinal Plants, Springer, 2023, pp. C1–C1.
  2. A. E. Al-Snafi, "Medicinal plants with antimicrobial activities (part 2): Plant based review," Scholars Academic Journal of Pharmacy, vol. 5, no. 6, pp. 208–239, 2016.
  3. M. P. Widrlechner, "History and utilization of Rosa damascena," Economic Botany, vol. 35, pp. 42–58, 1981.
  4. P. Hawkey, "The growing burden of antimicrobial resistance," Journal of Antimicrobial Chemotherapy, vol. 62, no. suppl_1, pp. i1–i9, 2008.
  5. M. Toner, "Revenge of the microbes: how bacterial resistance is undermining the antibiotic miracle," Emerging Infectious Diseases, vol. 11, no. 10, p. 1650, 2005.
  6. T. G. Turki, "Genetic Detection of iss, traT, and ompK36 Genes in Gram-Negative Bacteria Isolated from Different Infections in Najaf Province," Universal Publication Index e-Library, pp. 14–20, 2024.
  7. V. V. Tets and G. V. Tets, "Nutrient medium for cultivating bacteria," Google Patents, 2020.
  8. A. Zinedine and M. Faid, "Isolation and characterization of strains of Bifidobacteria with probiotic properties in vitro," World Journal of Dairy & Food Sciences, vol. 2, no. 1, pp. 28–34, 2007.
  9. R. A. Salman, M. W. M. Alzubaidy, and A. T. Tawfeeq, "Effect of Mirabilis Jalapa Plant Fortified with Nano-Quercetine to Accelerate Wound Healing in Vivo," Journal of Global Innovations in Agricultural Sciences, vol. 12, pp. 709–715, Aug. 2024.
  10. T. Nazli, A. Raheem, R. Raza, R. Khan, Z. A. Siddiqui, and G. Akhter, "The Chemical Constituents and Diverse Pharmacological Activities of Rosa damascena," Journal of Drug Discovery and Development, vol. 6, no. 2, pp. 1–7, Dec. 2022. [Online]. Available: https://medicaljournalshouse.com/index.php/JDrug-Discovery-Development/article/view/894.
  11. N. Thallaj, "Evaluation of antimicrobial activities and bioactive compounds of different extracts related to Syrian traditional products of damask rose (Rosa damascena)," Open Access Library Journal, vol. 7, no. 5, p. 1, 2020.
  12. M. Mahboubi, "Rosa damascena as holy ancient herb with novel applications," Journal of Traditional and Complementary Medicine, vol. 6, no. 1, pp. 10–16, 2016.
  13. K. Tano, M. K. Oulé, G. Doyon, R. W. Lencki, and J. Arul, "Comparative evaluation of the effect of storage temperature fluctuation on modified atmosphere packages of selected fruit and vegetables," Postharvest Biology and Technology, vol. 46, no. 3, pp. 212–221, 2007.
  14. N. Kumar, P. Bhandari, B. Singh, and S. S. Bari, "Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii," Food and Chemical Toxicology, vol. 47, no. 2, pp. 361–367, 2009.
  15. I. Kyriakidis, E. Vasileiou, Z. D. Pana, and A. Tragiannidis, "Acinetobacter baumannii Antibiotic Resistance Mechanisms," Pathogens, vol. 10, no. 3, p. 373, 2021. doi: 10.3390/pathogens10030373
  16. C. W. Song, J. M. Park, S. C. Chung, S. Y. Lee, and H. Song, "Microbial production of 2,3-butanediol for industrial applications," Journal of Industrial Microbiology & Biotechnology, vol. 46, no. 11, pp. 1583–1601, 2019.
  17. C. Sivaraj, R. Abhirami, M. Deepika, V. Sowmiya, K. Saraswathi, and P. Arumugam, "Antioxidant, antibacterial activities and GC-MS analysis of fresh rose petals aqueous extract of Rosa damascena Mill L," 2019.
  18. E. Denamur, O. Clermont, S. Bonacorsi, and D. Gordon, "The population genetics of pathogenic Escherichia coli," Nature Reviews Microbiology, vol. 19, no. 1, pp. 37–54, 2021.
  19. L. R. Mulcahy, V. M. Isabella, and K. Lewis, "Pseudomonas aeruginosa Biofilms in Disease," Microbial Ecology, vol. 68, no. 1, pp. 1–12, 2014. doi: 10.1007/s00248-013-0297-x
  20. A. L. Francis, S. K. R. Namasivayam, M. Kavisri, and M. Meivelu, "Anti-microbial efficacy and notable biocompatibility of Rosa damascena and Citrus sinensis biomass-derived metabolites," Biomass Conversion and Biorefinery, vol. 14, no. 19, pp. 24787–24807, Oct. 2024. doi: 10.1007/s13399-023-04439-8
  21. K. L. Wyres, M. M. C. Lam, and K. E. Holt, "Population genomics of Klebsiella pneumoniae," Nature Reviews Microbiology, vol. 18, no. 6, pp. 344–359, Jun. 2020. doi: 10.1038/s41579-019-0315-1
  22. S. D. Mahlen, "Serratia infections: from military experiments to current practice," Clinical Microbiology Reviews, vol. 24, no. 4, pp. 755–791, 2011.
  23. P. Ioannou and G. Vougiouklakis, "Infective endocarditis by Proteus species: a systematic review," Germs, vol. 10, no. 3, p. 229, 2020.
  24. M. Z. Jeddi, M. Yunesian, M. E. Gorji, N. Noori, M. R. Pourmand, and G. R. J. Khaniki, "Microbial evaluation of fresh, minimally-processed vegetables and bagged sprouts from chain supermarkets," Journal of Health, Population, and Nutrition, vol. 32, no. 3, p. 391, 2014.
  25. M. B. Tadhani and R. Subhash, "In vitro antimicrobial activity of Stevia rebaudiana Bertoni leaves," Tropical Journal of Pharmaceutical Research, vol. 5, no. 1, pp. 557–560, 2006.
  26. S. Calsamiglia, M. Busquet, P. Cardozo, L. Castillejos, and A. Ferret, "Invited review: Essential oils as modifiers of rumen microbial fermentation," Journal of Dairy Science, vol. 90, no. 6, pp. 2580–2595, 2007.
  27. M. K. Njogu, J. Matasyoh, and A. Kibor, "Isolation of four furoquinoline alkaloids from Teclea nobilis and their activity against Schistosoma mansoni miracidia," Journal of Biomedical and Pharmaceutical Research, vol. 3, pp. 87–93, 2014.
  28. P. Ajaybhan, Navneet, and A. Chauhan, "Evaluation of antimicrobial activity of six medicinal plants against dental pathogens," Report and Opinion, vol. 2, pp. 37–42, Jan. 2010.
  29. S. Calsamiglia, M. Busquet, P. W. Cardozo, L. Castillejos, and A. Ferret, "Invited review: Essential oils as modifiers of rumen microbial fermentation," Journal of Dairy Science, vol. 90, no. 6, pp. 2580–2595, 2007. doi: 10.3168/jds.2006-644
  30. S. Yilmaz and H. Fidan, "Pharmacological and toxicological profile of the Stachys lavandulifolia Vahl: a comprehensive review," Zeitschrift für Naturforschung C, 2024.
  31. L. Labban and N. Thallaj, "The medicinal and pharmacological properties of Damascene Rose (Rosa damascena): A review," International Journal of Herbal Medicine, vol. 8, pp. 33–37, 2020.
  32. R. M. H. Shoker, "A Review Article: The Importance of the Major Groups of Plant Secondary Metabolism Phenols, Alkaloids, and Terpenes," International Journal for Research in Applied Sciences and Biotechnology, 2020.
  33. M. H. Boskabady, M. N. Shafei, Z. Saberi, and S. Amini, "Pharmacological effects of Rosa damascena," Iranian Journal of Basic Medical Sciences, vol. 14, no. 4, p. 295, 2011.


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