Isolation and Identification of Bacteria Isolated from the Oral Cavity of Oral Leukoplakia

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Proceedings of International Conference on Applied Innovation in IT · 2025/06/27 · Vol. 13 · Issue 2 · pp. 833–837

Isolation and Identification of Bacteria Isolated from the Oral Cavity of Oral Leukoplakia

Saba Salman Kitab and Adawia Fadhil Abbas Alzubaidi

📄 Download PDF DOI: <a href="http://dx.doi.org/10.25673/120612">10.25673/120612</a>

Abstract

A potentially cancerous condition called oral leucoplakia (OL) is typified by white patches on the oral mucosa. Microbial colonization, especially by bacteria, has a role in the pathophysiology of OL by promoting inflammation and the advancement of the illness. In this study, bacterial species from the oral cavity of patients with OL will be isolated, identified, and compared to those found in healthy persons. From July to December 2024, samples were taken from dental clinics and specialty centers in the Diyala Governorate. Micrococcus luteus, Kocuria kristinae pneumonia, Streptococcus alactolyticus, Staphylococcus hominis, and Rothia dentocariosa were among the most commonly isolated bacterial species. The development and malignant transformation of oral leukoplakia may be facilitated by the presence of particular pathogenic microorganisms. Thirty of the 120 MDR bacterial samples showed antibacterial sensitivity, indicating a possible role in chronic inflammation and the development of disease. To better understand how these bacteria contribute to the pathophysiology of OL and create focused treatment plans, more investigation is needed.

Keywords

Oral Leukoplakias Bacterial Isolation Staphylococcus Aureus Oral Microbiome.

References

V. C. Carrard and I. van der Waal,"A clinical diagnosis of oral leukoplakia: a guide for dentists,” Medicina Oral, Patología Oral y Cirugía Bucal, vol. 23, pp. e59, 2018.
F. W. Mello, A. F. P. Miguel, K. L. Dutra, A. L. Porporatti, S. Warnakulasuriya, and E. N. S. Guerra,"Prevalence of oral potentially malignant disorders: a systematic review and meta-analysis,” J. Oral Pathol. Med., vol. 47, pp. 633–640, 2018.
S. Tovaru, M. Costache, P. Perlea, M. Caramida, C. Totan, I. Parlatescu, and S. Warnakulasuriya,"Oral leukoplakia: a clinicopathological study and malignant transformation,” Oral Dis., vol. 29, pp. 1454–1463, 2023.
M. B. C. Maymone, R. O. Greer, J. Kesecker, P. C. Sahitya, L. K. Burdine, A. D. Cheng, A. C. Maymone, and N. A. Vashi,"Premalignant and malignant oral mucosal lesions: clinical and pathological findings,” J. Am. Acad. Dermatol., vol. 81, pp. 59–71, 2019.
J. M. Aguirre-Urizar, I. Lafuente-Ibáñez de Mendoza, and S. Warnakulasuriya,"Malignant transformation of oral leukoplakia: systematic review and meta-analysis of the last 5 years,” Oral Dis., vol. 27, no. 8, pp. 1881–1895, 2021, doi: 10.1111/odi.13810.
A. S. Yaakop, A. Ahmad, F. Hussain, S. E. Oh, M. B. Alshammari, and R. Chauhan,"Domestic organic waste: a potential source to produce the energy via a single-chamber microbial fuel cell,” Int. J. Chem. Eng., 2023.
Clinical and Laboratory Standards Institute, Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Second Informational Supplement, CLSI Document M100-S22, Wayne, PA: CLSI, 2024.
L. Kotrbová, A. C. Lara, E. Corretto, J. Scharfen, V. Ulmann, K. Petříčková, and A. Chroňáková,"Evaluation and comparison of antibiotic susceptibility profiles of Streptomyces spp. clinical specimens revealed common and region-dependent resistance patterns,” Sci. Rep., vol. 12, no. 1, p. 9353, 2022.
X. Xiao, S. Liu, H. Deng, Y. Song, L. Zhang, and Z. Song,"Advances in the oral microbiota and rapid detection of oral infectious diseases,” Front. Microbiol., vol. 14, 2023, Art. no. 1121737, doi: 10.3389/fmicb.2023.1121737.
V. Anuta, M. T. Talianu, C. E. Dinu-Pirvu, M. V. Ghica, R. M. Prisada, M. G. Albu Kaya, et al.,"Molecular mapping of antifungal mechanisms accessing biomaterials and new agents to target oral candidiasis,” Int. J. Mol. Sci., vol. 23, p. 7520, 2022.
N. Atyeo, M. D. Rodriguez, B. Papp, and Z. Toth,"Clinical manifestations and epigenetic regulation of oral herpesvirus infections,” Viruses, vol. 13, p. 681, 2021, doi: 10.3390/v13040681.
R. D. Cannon,"Oral fungal infections: past, present, and future,” Front. Oral Health, vol. 3, p. 838639, 2022, doi: 10.3389/froh.2022.838639.
L. Chen, W. Liu, Q. Zhang, K. Xu, G. Ye, W. Wu, et al.,"RNA-based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak,” Emerg. Microbes Infect., vol. 9, pp. 313–319, 2020, doi: 10.1080/22221751.2020.1725399.
A. A. K. Rikabi, M. W. M. Alzubadiy, Z. H. Ali, H. M. Khudhair, and M. J. Abdulhasan, "Optimization of ecofriendly L-Fe/Ni nanoparticles prepared using extract of black tea leaves for removal of tetracycline antibiotics from groundwater by response surface methodology," South African Journal of Chemical Engineering, vol. 50, pp. 89–99, 2024. [Online]. Available: https://doi.org/10.1016/j.sajce.2024.07.007
L. Ciuffreda, H. Rodriguez-Perez, and C. Flores,"Nanopore sequencing and its application to the study of microbial communities,” Comput. Struct. Biotechnol. J., vol. 19, pp. 1497–1511, 2021, doi: 10.1016/j.csbj.2021.02.016.