Proceedings of International Conference on Applied Innovation in IT
2025/06/27, Volume 13, Issue 2, pp.577-584

Synthesis and Evaluation of New 1,3,4-Thiadiazole-Benzimidazole Hybrids as Potent Antibacterial Agents


Wejdan Khaled Harat and Muayad Ahmed Rdaiaan


Abstract: Owing to the rapid rise in antibiotic resistance, infectious diseases have become serious threat to public health. There is an urgent need to develop new antimicrobial agents with diverse chemical structures and novel mechanisms of action to overcome the resistance. In recent years 1,3,4-Thiadiazole benzimidazole hybrids have emerged as a new class of antimicrobial agents ,In the current study, we designed and synthesized six new 1,3,4-Thiadiazole -benzimidazole hybrids using nucleophilic substitution reaction between 2-mercapto-1H-benzimidazole (A) with Ethyl chloroacetate to produce compound (B), which then reaction with various 2-Amino-5-(substituted phenyl)-1,3,4-thiadiazole(C1-C6) to yielded target compounds (D1-D6) and evaluated them for their antimicrobial activity .Synthesized compounds were characterized and elucidated by IR, 1H and 13C-NMR, spectra. Subsequently The antibacterial activity of the final target compounds (D1-D6) was examined in vitro against four types of bacterial isolates: two gram-negative (Staphylococcus aureus and Bacillus subtilis) and two gram-positive (Pseudomonas aeruginosa and Escherichia coli). Compounds (D4 and D6) demonstrated promising pharmacological activity in vitro when compared to the standard antibacterial agent Tetracycline.

Keywords: Benzimidazole, 1,3,4-Thiadiazole, Antibacterial Activity, O-Phenylenediamine.

DOI: 10.25673/120545

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