Proceedings of International Conference on Applied Innovation in IT
2023/03/09, Volume 11, Issue 1, pp.133-141

Sensitivity Analysis of Enzyme-Substrate-Inhibitor Interaction Based on Nonlinear Dynamic Model


Vasyl Martsenyuk and Aleksandra Kłos-Witkowska


Abstract: The development of electrochemical biosensors is cutting-edge in current research in medicine, biology, and ecology. The modeling and study of the enzyme-substrate-inhibitor interaction are required for biosensor design. The aim of the paper is to construct a three-stage model for an electrochemical biosensor and to perform its sensitivity analysis. The research is based on the Morris method. The main results are concerning the comparative impact of the model parameters related to biochemical reaction rates on the dynamics of the changes of the concentrations of enzyme, substrate, inhibitor, three complexes, and the reaction product throughout three stages. The results have both theoretical and practical relevance as the model parameters studied come from a real case study of biosensors for alpha-chaconine.

Keywords: Morris Sensitivity Analysis, Enzyme, Substrate, Inhibitor, Michaelis-Menthen Model.

DOI: 10.25673/101929

Download: PDF

References:

  1. M. D. Morris, “Factorial sampling plans for preliminary computational experiments,” Technometrics, vol. 33, no. 2, pp. 161-174, May 1991, [Online]. Available: https://doi.org/10.1080/ 00401706.1991.10484804.
  2. J. P. Norton, “Selection of Morris trajectories for initial sensitivity analysis,” IFAC Proceedings Volumes, vol. 42, no. 10, pp. 670-674, 2009, 15th IFAC Symposium on System Identification, [Online]. Available: https://doi.org/10.3182/ 20090706-3-FR- 2004.00111.
  3. Q. Ge and M. Menendez, “Extending Morris method for qualitative global sensitivity analysis of models with dependent inputs,” Reliability Engineering System Safety, vol. 162, pp. 28-39, 2017, [Online]. Available: https://doi.org/10.1016/j.ress.2017.01.010.
  4. M. Jaxa-Rozen and J. Kwakkel, “Tree-based ensemble methods for sensitivity analysis of environmental models: A performance comparison with sobol and morris techniques,” Environmental Modelling Software, vol. 107, pp. 245-266, 2018, [Online]. Available: https://doi.org/10.1016/j.envsoft.2018. 06.011.
  5. V. Nicoulaud-Gouin and et al., “Sensitivity analysis in a radiological impact assessment of a nuclear power plant discharge. a comparison of the morris, spearman and sobol’ approaches,” Journal of Environmental Radioactivity, vol. 242, p. 106770, 2022, [Online]. Available: https://doi.org/10.1016/j.jenvrad.2021. 106770.
  6. X. Sun, B. Croke, A. Jakeman, and S. Roberts, “Benchmarking active subspace methods of global sensitivity analysis against variance-based sobol’ and morris methods with established test functions,” Environmental Modelling Software, vol. 149, p. 105310, 2022, [Online]. Available: https://doi.org/ 10.1016/j.envsoft.2022.105310.
  7. A. Janse van Rensburg, G. van Schoor, and P. van Vuuren, “Stepwise global sensitivity analysis of a physics-based battery model using the morris method and monte carlo experiments,” Journal of Energy Storage, vol. 25, p. 100875, 2019, [Online]. Available: https://doi.org/10.1016/ j.est.2019.100875.
  8. Y. Shen and M. Yarnold, “A novel sensitivity analysis of commercial building hybrid energy-structure performance,” Journal of Building Engineering, vol. 43, p. 102808, 2021, [Online]. Available: https://doi.org/10.1016/j.jobe.2021.102808.
  9. D. King and B. Perera, “Morris method of sensitivity analysis applied to assess the importance of input variables on urban water supply yield – a case study,” Journal of Hydrology, vol. 477, pp. 17-32, 2013, [Online]. Available: https://doi.org/10.1016/j.jhydrol. 2012.10.017.
  10. E. A. Baker, A. Cappato, S. Todeschini, L. Tamellini, G. Sangalli, A. Reali, and S. Manenti, “Combining the Morris method and multiple error metrics to assess aquifer characteristics and recharge in the lower Ticino Basin, in Italy,” Journal of Hydrology, vol. 614, pp. 128536, 2022, doi: 10.1016/j.jhydrol.2022. 128536.
  11. L. Gatel, C. Lauvernet, N. Carluer, S. Weill, J. Tournebize, and C. Paniconi, “Global evaluation and sensitivity analysis of a physically based flow and reactive transport model on a laboratory experiment,” Environmental Modelling Software, vol. 113, pp. 73-83, 2019, doi: 10.1016/j.envsoft. 2018.12.006.
  12. C. Wang, M. Peng, and G. Xia, “Sensitivity analysis based on Morris method of passive system performance under ocean conditions,” Annals of Nuclear Energy, vol. 137, pp. 107067, 2020, doi: 10.1016/j.anucene.2019.107067.
  13. B. P. English and et al., “Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited,” Nature Chemical Biology, vol. 2, no. 2, pp. 94, Dec. 2005, doi: 10.1038/nchembio759.
  14. B. Palsson, “On the dynamics of the irreversible Michaelis-Menten reaction mechanism,” Chemical Engineering Science, vol. 42, no. 3, pp. 447-458, 1987.
  15. J. Keener and J. Sneyd, Eds., Mathematical Physiology. Springer New York, 2009, doi: 10.1007/978-0-387-75847-3.
  16. S. Schnell, “Validity of the Michaelis-Menten equation - steady-state or reactant stationary assumption: that is the question,” FEBS Journal, vol. 281, no. 2, pp. 464-472, Nov. 2013, doi: 10.1111/febs.12564.
  17. W. D. Ristenpart, J. Wan, and H. A. Stone, “Enzymatic reactions in microfluidic devices: Michaelis-Menten kinetics,” Analytical Chemistry, vol. 80, no. 9, pp. 3270-3276, May 2008, doi: 10.1021/ac702469u.
  18. V. Martsenyuk, A. Klos-Witkowska, S. Dzyadevych, and A. Sverstiuk, “Nonlinear analytics for electrochemical biosensor design using enzyme aggregates and delayed mass action,” Sensors (Basel), vol. 22, no. 3, pp. 980, Jan. 2022.
  19. J. Ren, W. Zhang, and J. Yang, "Morris sensitivity analysis for hydrothermal coupling parameters of embankment dam: A case study," Mathematical Problems in Engineering, vol. 2019, pp. 1-11, Jun. 2019.
  20. “FAAI Job Hub – The Future is in Applied Artificial Intelligence 2022-1-PL01-KA220-HED-000088359,” http://faai.ath.edu.pl, [Accessed on Feb. 24, 2023].


    HOME

       - Call for Papers
       - Paper Submission
       - For authors
       - Important Dates
       - Conference Committee
       - Editorial Board
       - Reviewers
       - Last Proceedings


    PROCEEDINGS

       - Volume 12, Issue 1 (ICAIIT 2024)        - Volume 11, Issue 2 (ICAIIT 2023)
       - Volume 11, Issue 1 (ICAIIT 2023)
       - Volume 10, Issue 1 (ICAIIT 2022)
       - Volume 9, Issue 1 (ICAIIT 2021)
       - Volume 8, Issue 1 (ICAIIT 2020)
       - Volume 7, Issue 1 (ICAIIT 2019)
       - Volume 7, Issue 2 (ICAIIT 2019)
       - Volume 6, Issue 1 (ICAIIT 2018)
       - Volume 5, Issue 1 (ICAIIT 2017)
       - Volume 4, Issue 1 (ICAIIT 2016)
       - Volume 3, Issue 1 (ICAIIT 2015)
       - Volume 2, Issue 1 (ICAIIT 2014)
       - Volume 1, Issue 1 (ICAIIT 2013)


    PAST CONFERENCES

       ICAIIT 2024
         - Photos
         - Reports

       ICAIIT 2023
         - Photos
         - Reports

       ICAIIT 2021
         - Photos
         - Reports

       ICAIIT 2020
         - Photos
         - Reports

       ICAIIT 2019
         - Photos
         - Reports

       ICAIIT 2018
         - Photos
         - Reports

    ETHICS IN PUBLICATIONS

    ACCOMODATION

    CONTACT US

 

DOI: http://dx.doi.org/10.25673/115729


        

         Proceedings of the International Conference on Applied Innovations in IT by Anhalt University of Applied Sciences is licensed under CC BY-SA 4.0


                                                   This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License


           ISSN 2199-8876
           Publisher: Edition Hochschule Anhalt
           Location: Anhalt University of Applied Sciences
           Email: leiterin.hsb@hs-anhalt.de
           Phone: +49 (0) 3496 67 5611
           Address: Building 01 - Red Building, Top floor, Room 425, Bernburger Str. 55, D-06366 Köthen, Germany

        site traffic counter

Creative Commons License
Except where otherwise noted, all works and proceedings on this site is licensed under Creative Commons Attribution-ShareAlike 4.0 International License.